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frequency separation for retouching backgrounds - photo tutorials

frequency separation for retouching backgrounds - photo tutorials

Frequency separation is a powerful technique. Photographers think of frequency separation as a skin retouching tool, but it is so much more. I often use usefrequency separation for retouching backgrounds. Affinity Photo includes frequency separation built right in which is convenient. In this retouching walk through, I show you how to use it to fix sections of the background in a natural way.

One of my upcoming brides was brave and embraced the idea of an underwater bridal session. We took over a friends pool and started shooting. Underwater photography adds a large number of complications. Most water, even the cleanest, isnt really that clean. There are always particles of dust and grit floating around. When you toss in a model, a photographer, a background and a bunch of other stuff and then swim around, the water gets murky quickly.

I always try to be as close to the model as I can when shooting under water. We were shooting on a 16-35mm lens and I was zoomed all the way out to 16mm. This creates distortion but that is a trade off I accept. It also brings in a lot of background Id like to avoid.

Even with our 15 wide black muslin backdrop, the sides of the pool were visible. They broke the mood of the photo. It takes the viewer out of a mysterious situation and puts them into a pool. That isnt what I wanted. Therefore, I retouched the image to remove the unwanted visible pool on the edges.

Frequency separation separates high frequency changes (small details) from low frequency changes (the overall tone and hue of areas of the image). By separating out details, you can work independently on different aspects of the image.

In this image I needed to alter the background to remove the obvious sections of pool wall. Because the mid-ground had a lot of fine details, such as the flecks lit up in the water, simple cloning or in painting would be a nightmare. With frequency separation, I was able to work independently on the background tone and hue without having to worry about the fore and mid-ground water.

He is a self taught experiential learner who is addicted to the possibilities that new (to him) gear open up. He loves to share the things he has worked out. Andrew started with a passion for landscape and night photography and quickly branched out to work in just about every form of photography. He is an ex-software developer with extensive experience in the IT realm.

He is a self taught experiential learner who is addicted to the possibilities that new (to him) gear open up. He loves to share the things he has worked out. Andrew started with a passion for landscape and night photography and quickly branched out to work in just about every form of photography. He is an ex-software developer with extensive experience in the IT realm.

AzulOx Workshops are Austin Photography Workshops. Our goal is to teach, demonstrate and nurture specific photography skills with cameras and gear in your hands. Workshops are limited to a small number of participants to maximize your learning time and access to the instructors.

high-resolution crispr screens reveal fitness genes and genotype-specific cancer liabilities - sciencedirect

high-resolution crispr screens reveal fitness genes and genotype-specific cancer liabilities - sciencedirect

Identification of 5-fold more fitness genes in human cells than previously observedCore fitness genes are highly enriched for ancient protein complexesContext-specific fitness genes illuminate biological differences between cell typesDistinct genetic signatures can be used to predict differential drug response

The ability to perturb genes in human cells is crucial forelucidating gene function and holds great potential for finding therapeutic targets for diseases such as cancer. To extend the catalog of human core and context-dependent fitness genes, we have developed a high-complexity second-generation genome-scale CRISPR-Cas9 gRNA library and applied it to fitness screens in five human cell lines. Using an improved Bayesian analytical approach, we consistently discover 5-fold more fitness genes than were previously observed. We present a list of 1,580 human core fitness genes and describe their general properties. Moreover, we demonstrate that context-dependent fitness genes accurately recapitulate pathway-specificgenetic vulnerabilities induced by known oncogenes and reveal cell-type-specific dependencies for specific receptor tyrosine kinases, even in oncogenic KRAS backgrounds. Thus, rigorous identification of human cell line fitness genes using a high-complexity CRISPR-Cas9 library affords a high-resolution view of the genetic vulnerabilities of a cell.

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